Ethanol-induced Translocation of cAMP-dependent Protein Kinase to the Nucleus: MECHANISM AND FUNCTIONAL CONSEQUENCES
A Constantinescu, I Diamond, AS Gordon - Journal of Biological Chemistry, 1999 - ASBMB
Ethanol induces translocation of the catalytic subunit (Cα) of cAMP-dependent protein
kinase (PKA) from the Golgi area to the nucleus in NG108–15 cells. Ethanol also induces
translocation of the RIIβ regulatory subunit of PKA to the nucleus; RI and Cβ are not
translocated. Nuclear PKA activity in ethanol-treated cells is no longer regulated by cAMP.
Gel filtration and immunoprecipitation analysis confirm that ethanol blocks the reassociation
of Cα with RII but does not induce dissociation of these subunits. Ethanol also reduces …
kinase (PKA) from the Golgi area to the nucleus in NG108–15 cells. Ethanol also induces
translocation of the RIIβ regulatory subunit of PKA to the nucleus; RI and Cβ are not
translocated. Nuclear PKA activity in ethanol-treated cells is no longer regulated by cAMP.
Gel filtration and immunoprecipitation analysis confirm that ethanol blocks the reassociation
of Cα with RII but does not induce dissociation of these subunits. Ethanol also reduces …
