Clinical relevance of the cagA, vacA, and iceA status of Helicobacter pylori
LJ van Doorn, C Figueiredo, R Sanna, A Plaisier… - Gastroenterology, 1998 - Elsevier
LJ van Doorn, C Figueiredo, R Sanna, A Plaisier, P Schneeberger, W de Boer, W Quint
Gastroenterology, 1998•ElsevierBackground & Aims: Clinical outcome of Helicobacter pylori infection may be associated
with specific virulence-associated bacterial genotypes. The aim of this study was to assess
the relationships between H. pylori cagA, vacA, and iceA status and severity of disease.
Methods: Gastric biopsy specimens from 94 patients in The Netherlands were analyzed by
polymerase chain reaction and reverse hybridization. Results: cagA was present in 63
(67%) of 94 cases and was associated with peptic ulcer disease (P= 0.0019). vacA …
with specific virulence-associated bacterial genotypes. The aim of this study was to assess
the relationships between H. pylori cagA, vacA, and iceA status and severity of disease.
Methods: Gastric biopsy specimens from 94 patients in The Netherlands were analyzed by
polymerase chain reaction and reverse hybridization. Results: cagA was present in 63
(67%) of 94 cases and was associated with peptic ulcer disease (P= 0.0019). vacA …
Background & Aims
Clinical outcome of Helicobacter pylori infection may be associated with specific virulence-associated bacterial genotypes. The aim of this study was to assess the relationships between H. pylori cagA, vacA, and iceA status and severity of disease.
Methods
Gastric biopsy specimens from 94 patients in The Netherlands were analyzed by polymerase chain reaction and reverse hybridization.
Results
cagA was present in 63 (67%) of 94 cases and was associated with peptic ulcer disease (P = 0.0019). vacA genotypes s1a/m1, s1a/m2, s1b/m1, s1b/m2, and s2/m2 were found in 36.2%, 23.4%, 2.1%, 5.3%, and 20.2%, respectively. Ten isolates (10.6%) contained multiple vacA genotypes. The presence of peptic ulcers was associated with type s1 strains (P = 0.0006) but not with the m type (P = 0.2035). cagA and vacA s1 were strongly associated (P < 10−5). iceA1 was found in 53 (56.4%) and iceA2 in 25 (26.6%) of the 94 cases. In 14 isolates (14.9%), both iceA alleles were found, and 2 (2.1%) were negative for both iceA1 and iceA2. iceA1 was also associated with peptic ulcer disease (P = 0.0042). The iceA allelic type was independent of the cagA and vacA status.
Conclusions
vacA s1, cagA, and iceA1 are markers of H. pylori strains that are more likely to lead to ulcer disease. GASTROENTEROLOGY 1998;115:58-66
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