Expression of the murine cytomegalovirus glycoprotein H by recombinant vaccinia virus
M Rapp, P Lučin, M Messerle… - Journal of general …, 1994 - microbiologyresearch.org
Journal of general virology, 1994•microbiologyresearch.org
The sequence of the gene encoding glycoprotein H (gH) of murine cytomegalovirus (MCMV)
strain Smith was determined and compared with the sequence of the gH of MCMV strain
K181. Transcriptional analysis showed that gH is encoded by a large mRNA of 5· 0 kb,
which is synthesized late in infection. A recombinant vaccinia virus expressing the MCMV
gH open reading frame was constructed (Vac-gH). Anti-MCMV serum precipitated a protein
of 87K from Vac-gH-infected cells. Reactivity with a monoclonal antibody showed the identity …
strain Smith was determined and compared with the sequence of the gH of MCMV strain
K181. Transcriptional analysis showed that gH is encoded by a large mRNA of 5· 0 kb,
which is synthesized late in infection. A recombinant vaccinia virus expressing the MCMV
gH open reading frame was constructed (Vac-gH). Anti-MCMV serum precipitated a protein
of 87K from Vac-gH-infected cells. Reactivity with a monoclonal antibody showed the identity …
The sequence of the gene encoding glycoprotein H (gH) of murine cytomegalovirus (MCMV) strain Smith was determined and compared with the sequence of the gH of MCMV strain K181. Transcriptional analysis showed that gH is encoded by a large mRNA of 5·0 kb, which is synthesized late in infection. A recombinant vaccinia virus expressing the MCMV gH open reading frame was constructed (Vac-gH). Anti-MCMV serum precipitated a protein of 87K from Vac-gH-infected cells. Reactivity with a monoclonal antibody showed the identity of the MCMV gH with a 87K envelope glycoprotein described previously by Loh and Qualtiere. Immunization of mice with the Vac-gH recombinant gave rise to an anti-gH serum, which neutralized MCMV without complement in vitro.
Microbiology Research