Reversible HLA multimers (Streptamers) for the isolation of human cytotoxic T lymphocytes functionally active against tumor-and virus-derived antigens
J Neudorfer, B Schmidt, KM Huster, F Anderl… - Journal of …, 2007 - Elsevier
The development of MHC/peptide multimers has facilitated the visualization and purification
of antigen-specific T cells. However, the persistence of multimers leads to prolonged T cell
receptor signaling and subsequently to altered T-cell function. We have recently developed
a new type of MHC/peptide multimers, which can be dissociated from the T cell. Herein, we
have generated and tested for the first time reversible HLA/peptide multimers, termed
Streptamers, for the isolation of human T cells. The Streptamer technique demonstrates the …
of antigen-specific T cells. However, the persistence of multimers leads to prolonged T cell
receptor signaling and subsequently to altered T-cell function. We have recently developed
a new type of MHC/peptide multimers, which can be dissociated from the T cell. Herein, we
have generated and tested for the first time reversible HLA/peptide multimers, termed
Streptamers, for the isolation of human T cells. The Streptamer technique demonstrates the …