Isolation and in vitro expansion of human colonic stem cells
P Jung, T Sato, A Merlos-Suárez, FM Barriga… - Nature medicine, 2011 - nature.com
Nature medicine, 2011•nature.com
Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell
surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different
cell types from human colon mucosa biopsies. The highest EPHB2 surface levels
correspond to epithelial colonic cells with the longest telomeres and elevated expression of
intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate
the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an …
surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different
cell types from human colon mucosa biopsies. The highest EPHB2 surface levels
correspond to epithelial colonic cells with the longest telomeres and elevated expression of
intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate
the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an …
Abstract
Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an undifferentiated and multipotent population.
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